mouse anti notch ecd  (Developmental Studies Hybridoma Bank)

Journal: bioRxiv

Article Title: Absence of EOGT Precludes Defective Development in Fringe-null Mouse Intestine

doi: 10.64898/2026.02.10.705133

Figure Lengend Snippet: O-Glycans of EGF repeats and consequences of their removal. (A) Diagram depicting the EGF repeats of NOTCH1, NOTCH2, DLL1 and DLL4 extracellular domains. The consensus sequence recognized by the glycosyltransferase that transfers a Fuc, Glc or GlcNAc to Ser/Thr in an EGF repeat is given on the left and the sequence of sugars that may occur at each site is given on the right. The Controls column shows the full sugar extension that may occur at each site with the enzyme(s) that would be responsible for transfer [ , - ]. The other columns show the sequences remaining after deletion of Lfng alone ( Lfng cKO) or all three Fringes together (LMR tKO) or all three Fringes in an Eogt -null background (ELMR qKO). Parentheses indicate potential additions. Shaded sugars in Lfng cKO reflect potential addtion by MFNG and/or RFNG. (B, C) Body weight (B) and small intestine (SI) length (C) in control and experimental mice (n ≥ 7 mice per group). P values from one-way ANOVA followed by Tukey’s multiple comparisons test ** P < 0.01, *** P < 0.001.

Article Snippet: Cells were assessed for NOTCH1 surface expression with anti-NOTCH1 extracellular domain (ECD) antibody (Ab AF5267; R&D Systems, Inc., Minneapolis, MN) and binding of Notch ligands DLL1-Fc (R&D Systems, Inc.) and DLL4-Fc (Acro Biosystems, Newark, DE).

Techniques: Sequencing, Control

Journal: bioRxiv

Article Title: Absence of EOGT Precludes Defective Development in Fringe-null Mouse Intestine

doi: 10.64898/2026.02.10.705133

Figure Lengend Snippet: Notch ligand binding to ISC in crypts. (A-C) Flow cytometry profiles of anti-NOTCH1 ECD Ab, DLL1-Fc and DLL4-Fc binding to ISC from Control, LMR tKO and ELMR qKO crypts. Pink profiles show Ab to NOTCH1 ECD or Notch ligand-Fc binding, blue profiles show 2° antibody non-specific binding. (D) MFI for Ab to NOTCH1 ECD, DLL1-Fc and DLL4-Fc binding to LMR tKO and ELMR qKO normalized to MFI of Control in each of six experiments performed over 6 days. MFI values varied between experiments so each experiment was analyzed separately. One-way ANOVA followed by Tukey’s multiple comparisons test was used to determine P values.

Article Snippet: Cells were assessed for NOTCH1 surface expression with anti-NOTCH1 extracellular domain (ECD) antibody (Ab AF5267; R&D Systems, Inc., Minneapolis, MN) and binding of Notch ligands DLL1-Fc (R&D Systems, Inc.) and DLL4-Fc (Acro Biosystems, Newark, DE).

Techniques: Ligand Binding Assay, Flow Cytometry, Binding Assay, Control